Map-based cloning of a protein kinase gene conferring disease resistance in tomato

Science. 1993 Nov 26;262(5138):1432-6. doi: 10.1126/science.7902614.

Abstract

The Pto gene in tomato confers resistance to races of Pseudomonas syringae pv. tomato that carry the avirulence gene avrPto. A yeast artificial chromosome clone that spans the Pto region was identified and used to probe a leaf complementary DNA (cDNA) library. A cDNA clone was isolated that represents a gene family, at least six members of which genetically cosegregate with Pto. When susceptible tomato plants were transformed with a cDNA from this family, they were resistant to the pathogen. Analysis of the amino acid sequence revealed similarity to serine-threonine protein kinases, suggesting a role for Pto in a signal transduction pathway.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Chromosomes, Artificial, Yeast
  • Cloning, Molecular*
  • DNA, Complementary / genetics
  • Genes, Plant*
  • Molecular Sequence Data
  • Multigene Family*
  • Plant Diseases / genetics*
  • Plant Proteins*
  • Polymorphism, Restriction Fragment Length
  • Protein Serine-Threonine Kinases / chemistry
  • Protein Serine-Threonine Kinases / genetics*
  • Protein Serine-Threonine Kinases / metabolism
  • Pseudomonas / pathogenicity
  • Signal Transduction
  • Vegetables / enzymology
  • Vegetables / genetics*
  • Vegetables / microbiology
  • Virulence

Substances

  • DNA, Complementary
  • Plant Proteins
  • Pto protein, Lycopersicon
  • Protein Serine-Threonine Kinases

Associated data

  • GENBANK/U02271