Gluten stimulation of coeliac mucosa in vitro induces activation (CD25) of lamina propria CD4+ T cells and macrophages but no crypt-cell hyperplasia

Scand J Immunol. 1993 Dec;38(6):581-90. doi: 10.1111/j.1365-3083.1993.tb03245.x.


Jejunal biopsy specimens from 10 patients with treated coeliac disease and seven non-coeliac controls were challenged in vitro with peptic-tryptic gluten digest. Mucosal T cells were examined in situ by three-colour immunofluorescence staining for expression of the activation marker CD25 (the p55 alpha-chain of interleukin-2 receptor) and the nuclear proliferation marker revealed by monoclonal antibody Ki-67. Intraepithelial T cells expressed CD25 rarely whereas the proportion of activated lamina propria T cells increased (P < 0.002) from median 2.8% (cultured with 20% fetal calf serum alone for 24-48 h) to 10.0% after 24 h with gluten (n = 10; range 1.1-17.4%) and to 10.4% after 48 h (n = 7; range 1.4-17.5%). Such gluten-induced increase of CD25+ T cells was not observed in specimens from non-coeliac control subjects. Crypt-cell hyperplasia and T-cell proliferation (Ki-67+) were observed neither in the coeliac nor in the control mucosae after gluten stimulation. Three-colour staining combining a polyclonal antibody reagent to CD3 and a monoclonal antibody to CD25 with a monoclonal antibody to CD45R0, CD4, CD8, the p75 beta-chain of interleukin-2 receptor, integrin alpha E beta 7, or HLA-DR showed that most of the CD25+ T cells (> 90%) were CD4+CD8-, co-expressed CD45R0 and the p75 beta-chain, and often also the integrin alpha E beta 7 but not HLA-DR. In addition to these activated T cells, a dominating population of CD25+CD3-CD4+ subepithelial pan-HLA-class II+ macrophages (CD68+) with variable expression of the p75 beta-chain was often induced by gluten challenge.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Autoantigens / immunology
  • CD4-Positive T-Lymphocytes / immunology*
  • Celiac Disease / immunology*
  • Cells, Cultured
  • Female
  • Fluorescent Antibody Technique
  • Glutens / immunology*
  • Humans
  • Hyperplasia
  • Intestinal Mucosa / immunology*
  • Intestinal Mucosa / pathology*
  • Jejunum / immunology*
  • Jejunum / pathology
  • Lymphocyte Activation*
  • Macrophages / immunology*
  • Male
  • Middle Aged
  • Nuclear Proteins / biosynthesis
  • Proliferating Cell Nuclear Antigen
  • Receptors, Interleukin-2 / biosynthesis*


  • Autoantigens
  • Nuclear Proteins
  • Proliferating Cell Nuclear Antigen
  • Receptors, Interleukin-2
  • Glutens