Impairment of T cell receptor-dependent stimulation in CD4+ lymphocytes after contact with membrane-bound HIV-1 envelope glycoprotein

Virology. 1994 Jan;198(1):360-5. doi: 10.1006/viro.1994.1042.

Abstract

A CD4+ human T cell clone (SPB21) or primary blood mononuclear cells were grown in the presence of HeLa cells stably expressing functional human immunodeficiency virus type 1 envelope glycoprotein complexes at their surface. After a short cocultivation, SPB21 cells lost their ability to proliferate in response to T cell receptor (TCR) stimulations and died by apoptosis, whereas interleukin-2 stimulation was still effective. Incubation with soluble monomeric gp120 did not alter TCR responsiveness. A selective decrease in the proportion of CD4+ cells was also observed among primary lymphocytes after cocultivation and OKT3 stimulation. We propose that binding of oligomeric membrane-bound envelope glycoprotein induces a multimerization of CD4 molecules that impairs normal TCR stimulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis
  • CD4-Positive T-Lymphocytes / immunology*
  • Cell Communication
  • Clone Cells
  • Gene Products, env / immunology*
  • HIV Envelope Protein gp120 / immunology
  • HIV Envelope Protein gp160
  • HIV-1 / immunology*
  • HeLa Cells
  • Humans
  • Lymphocyte Activation*
  • Protein Precursors / immunology
  • Receptors, Antigen, T-Cell / immunology*
  • Recombinant Proteins / immunology

Substances

  • Gene Products, env
  • HIV Envelope Protein gp120
  • HIV Envelope Protein gp160
  • Protein Precursors
  • Receptors, Antigen, T-Cell
  • Recombinant Proteins