Induction of a proteinase inhibitor II-class gene by auxin in tomato roots

Plant Mol Biol. 1993 Dec;23(5):1005-14. doi: 10.1007/BF00021815.


A cDNA clone corresponding to a transcript that was induced by auxin in tomato seedling roots encodes a 223 amino acid protein belonging to the proteinase inhibitor II family of tomato and potato. The TR8 open reading frame has an N-terminal region with characteristics of a signal peptide and three copies of a 64 amino acid segment that is also present in multiple copies in other PI-II family members. Comparison to other serine proteinase inhibitors indicates that all three domains are trypsin-specific. Transcripts homologous to TR8 increased in abundance within 24 h of auxin treatment and continued to increase through 72 h, at which point the induction was approximately ten-fold. Auxin induction was observed in roots and hypocotyls, but not in cotyledons or epicotyls. Induction may therefore be correlated with lateral and adventitious root initiation, which occurs over a similar time frame in the same tissues. RFLPs detected with the TR8 probe were mapped to the long arm of chromosome 11.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Consensus Sequence
  • DNA, Complementary / genetics
  • Gene Expression Regulation / drug effects
  • Genes, Plant*
  • Indoleacetic Acids / pharmacology*
  • Molecular Sequence Data
  • Plant Proteins / genetics
  • Polymorphism, Restriction Fragment Length
  • Protease Inhibitors / metabolism*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Vegetables / genetics*


  • DNA, Complementary
  • Indoleacetic Acids
  • Plant Proteins
  • Protease Inhibitors

Associated data

  • GENBANK/L21194