Injection of digitally synthesized synaptic conductance transients to measure the integrative properties of neurons

J Neurosci Methods. 1993 Sep;49(3):157-65. doi: 10.1016/0165-0270(93)90119-c.


A novel technique was developed for injecting a time-varying conductance into a neuron, to allow quantitative measurement of the processing of synaptic inputs. In current-clamp recording mode, the membrane potential was sampled continuously and used to calculate and update the level of injected current within 60 microseconds, using a real-time computer, so as to mimic the electrical effect of a given conductance transient. Cellular responses to synthetic conductance transients modelled on the fast (non-N-methyl-D-aspartate) phase of the glutamatergic postsynaptic potential were measured in cultured rat hippocampal neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Computer Simulation*
  • Electric Conductivity
  • Electric Stimulation / methods*
  • Electronic Data Processing
  • Glutamates / physiology
  • Glutamic Acid
  • Hippocampus / cytology
  • Hippocampus / physiology
  • Injections
  • Models, Neurological*
  • Neurons / physiology*
  • Rats
  • Reaction Time
  • Synapses / physiology*


  • Glutamates
  • Glutamic Acid