Abstract
The complete cDNA sequence of a human isolog of the yeast suil translation initiation factor gene was obtained by assembling over 40 expressed sequence tags (ESTs) for this gene obtained from a variety of tissue-specific cDNA libraries. The human suilisol gene product is a 113 amino-acid polypeptide similar to proteins known from yeast, rice, mosquito, and Methanococcus. The identification of suilisol illustrates the utility of assemblies of independent ESTs for deriving full-length cDNA sequences for new human genes.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Culicidae / genetics
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DNA, Complementary / analysis
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DNA, Complementary / chemistry
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DNA, Complementary / genetics*
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Eukaryotic Initiation Factor-1
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Fungal Proteins / genetics*
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Gene Expression
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Gene Library
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Genes, Fungal*
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Hominidae / genetics*
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Humans
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Methanococcus / genetics
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Molecular Sequence Data
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Open Reading Frames
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Oryza / genetics
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Peptide Initiation Factors / genetics*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
Substances
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DNA, Complementary
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Eukaryotic Initiation Factor-1
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Fungal Proteins
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Peptide Initiation Factors
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SUI1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins