Phosphoinositide phosphorylation and shape changes produced by phosmet-oxon in human erythrocytes

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1993 Oct;106(2):561-6. doi: 10.1016/0742-8413(93)90179-o.

Abstract

1. "In vitro" incubation of red blood cells with phosmetoxon induced crenated and invaginated forms. 2. [32P] phosphate incorporation was greater in membranes from erythrocytes exposed to 300 nM phosmetoxon for 10 min than in control cells. 3. The highest incorporation was for phosphatidylinositol (PI), followed by phosphatidylinositol phosphate (PIP) and phosphatidylinositolbiphosphate (PIP2). 4. An activation of phosphatidylinositol (PI) kinase was detected with 150 and 300 nM of the pesticide, while there was no change in poliphosphoinositides (PPI) phosphodiesterase activity. 5. Results suggest an association between changes in PI kinase activity, the phosphorylation cycle of phosphatidylinositols and alterations in erythrocyte morphology induced by phosmetoxon.

MeSH terms

  • 1-Phosphatidylinositol 4-Kinase
  • Cell Size / drug effects
  • Erythrocytes / cytology
  • Erythrocytes / drug effects*
  • Erythrocytes / metabolism
  • Humans
  • In Vitro Techniques
  • Lipids / analysis
  • Microscopy, Electron, Scanning
  • Phosmet / metabolism
  • Phosmet / pharmacology*
  • Phosphatidylinositols / metabolism*
  • Phosphoinositide Phospholipase C
  • Phosphoric Diester Hydrolases / analysis
  • Phosphorylation / drug effects
  • Phosphotransferases (Alcohol Group Acceptor) / analysis

Substances

  • Lipids
  • Phosphatidylinositols
  • Phosphotransferases (Alcohol Group Acceptor)
  • 1-Phosphatidylinositol 4-Kinase
  • Phosphoric Diester Hydrolases
  • Phosphoinositide Phospholipase C
  • Phosmet