Differential expression of PKC alpha and PKC beta isozymes in CD4+, CD8+ and CD4+/CD8+ double positive human T cells

FEBS Lett. 1994 Feb 7;338(3):339-42. doi: 10.1016/0014-5793(94)80295-5.


Using specific monoclonal and polyclonal antibodies, we have analyzed protein kinase C alpha and beta isozyme expression in human T cells from peripheral blood (PB) and from thymus. While the PKC beta isozyme was present in all T cell sub-types isolated from both PB and thymus, the alpha isozyme was found only in single positive CD4+ thymocytes, in PB-CD4+ lymphocytes and in PB-CD8+ T cells from several donors. It was absent from both, CD8+ and double positive CD4+/CD8+ thymocytes. These results show that PKC alpha and -beta are differentially regulated during intra-thymic development and suggest that PKC alpha plays a specific role in helper T cell function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD4 Antigens
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / metabolism
  • CD8 Antigens
  • Cells, Cultured
  • Humans
  • Isoenzymes / biosynthesis*
  • Protein Kinase C / biosynthesis*
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocyte Subsets / metabolism*
  • Thymus Gland / cytology


  • CD4 Antigens
  • CD8 Antigens
  • Isoenzymes
  • Protein Kinase C