Blood vessel invasion and expression of sialyl Lewisx and proliferating cell nuclear antigen in stage I non-small cell lung cancer. Relation to postoperative recurrence

Cancer. 1994 Feb 15;73(4):1177-83. doi: 10.1002/1097-0142(19940215)73:4<1177::aid-cncr2820730409>;2-0.


Background: Recurrence in Stage I non-small cell lung cancer was examined with respect to vascular invasion and the immunohistochemical expression of sialyl-dimeric Lewisx (SLX) and proliferating cell nuclear antigen (PCNA).

Methods: One hundred twenty-eight patients with Stage I non-small cell lung cancer who had a curative resection were the subjects of this study. Using tumor tissues fixed in formaldehyde solution, blood vessel invasion (BVI) and lymphatic invasion stained with Victoria blue-hematoxylin and eosin and the immunohistochemical expression of SLX and PCNA were retrospectively studied with respect to postoperative recurrence.

Results: By univariate analysis, BVI and SLX and PCNA expression were significantly important factors of disease-free survival (P < 0.01). The disease-free survival of the patients with both BVI and SLX expression was significantly shorter than that of the patients with BVI but negative SLX expression (P < 0.02). In 35 patients with recurrence, tumors with PCNA expression showed a significantly shorter time to recurrence compared with tumors without PCNA expression (P < 0.01).

Conclusions: BVI and SLX expression may be important determinants of recurrence. PCNA may be a determinant of time to recurrence.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, Neoplasm / analysis*
  • Biomarkers, Tumor / analysis*
  • Carcinoma, Non-Small-Cell Lung / blood supply
  • Carcinoma, Non-Small-Cell Lung / immunology*
  • Carcinoma, Non-Small-Cell Lung / mortality
  • Carcinoma, Non-Small-Cell Lung / pathology
  • Carcinoma, Non-Small-Cell Lung / secondary*
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Lewis Blood Group Antigens / analysis*
  • Lung Neoplasms / blood supply
  • Lung Neoplasms / immunology*
  • Lung Neoplasms / mortality
  • Lung Neoplasms / pathology*
  • Lymphatic Metastasis / immunology
  • Male
  • Middle Aged
  • Neoplasm Invasiveness / immunology
  • Neoplasm Recurrence, Local / immunology
  • Nuclear Proteins / analysis*
  • Proliferating Cell Nuclear Antigen
  • Retrospective Studies
  • Survival Analysis


  • Antigens, Neoplasm
  • Biomarkers, Tumor
  • Lewis Blood Group Antigens
  • Nuclear Proteins
  • Proliferating Cell Nuclear Antigen