Update on proliferation-associated antibodies applicable to formalin-fixed paraffin-embedded tissue and their clinical applications

Histochem J. 1993 Dec;25(12):843-53.

Abstract

This review provides an update on proliferation-associated antibodies applicable to immunohistochemical techniques in formalin-fixed paraffin-embedded tissue. New insights into proliferating cell nuclear antigen (PCNA) and antibodies to PCNA are presented. The characterization of the protein recognized by the Ki-67 antibody has enabled production of a new range of antibodies (monoclonal and polyclonal) which have immunostaining profiles similar to those of the original antibody. A new proliferation-associated antibody, KiS1, is described. The clinical applications of antibodies to PCNA in human material are summarized, and the limitations of these studies are discussed.

Publication types

  • Review

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Antigens, Neoplasm / analysis*
  • Antigens, Neoplasm / immunology
  • Biomarkers / analysis*
  • Biomarkers, Tumor / analysis
  • Cell Division*
  • DNA Topoisomerases, Type II
  • DNA-Binding Proteins
  • Humans
  • Immunohistochemistry
  • Ki-67 Antigen
  • Neoplasm Proteins / analysis*
  • Neoplasm Proteins / immunology
  • Nuclear Proteins / analysis*
  • Nuclear Proteins / immunology
  • Poly-ADP-Ribose Binding Proteins
  • Proliferating Cell Nuclear Antigen

Substances

  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Biomarkers
  • Biomarkers, Tumor
  • DNA-Binding Proteins
  • Ki-67 Antigen
  • Neoplasm Proteins
  • Nuclear Proteins
  • Poly-ADP-Ribose Binding Proteins
  • Proliferating Cell Nuclear Antigen
  • DNA Topoisomerases, Type II
  • TOP2A protein, human