An African Swine Fever Virus Gene With Similarity to the T-lymphocyte Surface Antigen CD2 Mediates Hemadsorption

Virology. 1994 Mar;199(2):463-8. doi: 10.1006/viro.1994.1146.

Abstract

An open reading frame, LMW8-DR, in the African swine fever virus (ASFV) genome possesses striking similarity to the lymphocyte membrane antigen CD2. All characterized CD2 domains, including the amino-terminal signal sequence, IgV, hinge, IgC2, stalk, transmembrane, and proline-rich carboxy cytoplasmic domains, are highly conserved in the ASFV gene. Critical residues for the binding of the lymphocyte function-associated antigen (LFA-3) and CD59 and for T-cell activation are also partially conserved. LMW8-DR is actively transcribed in ASFV-infected swine macrophages and Vero cells at late times in the infection cycle and Vero and COS cells transiently expressing the LMW8-DR open reading frame hemadsorbed swine red blood cells. The structural and functional similarities of LMW8-DR to CD2, a protein that is involved in cell-cell adhesion and immune response modulation, suggest a possible role in the pathogenesis of ASFV infection.

Publication types

  • Comparative Study

MeSH terms

  • African Swine Fever Virus / genetics*
  • Amino Acid Sequence
  • Animals
  • Antigens, Differentiation, T-Lymphocyte / chemistry*
  • Base Sequence
  • CD2 Antigens
  • Cells, Cultured
  • Conserved Sequence / genetics
  • Genes, Viral / physiology*
  • Hemadsorption / genetics*
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Receptors, Immunologic / chemistry*
  • Sequence Homology, Amino Acid
  • Vero Cells
  • Viral Proteins / chemistry
  • Viral Proteins / genetics*
  • Viral Proteins / physiology

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • CD2 Antigens
  • LMW8-DR protein, African swine fever virus
  • Receptors, Immunologic
  • Viral Proteins

Associated data

  • GENBANK/L15337