Isolation and mapping of a cosmid clone containing the human NAT2 gene

Biochem Biophys Res Commun. 1994 Feb 28;199(1):52-5. doi: 10.1006/bbrc.1994.1192.


The NAT2 gene encodes for a polymorphic arylalkylamine N-acetyltransferase and thus accounts for the human N-acetylation polymorphism. By a NAT2-specific primer set we have screened a human chromosome 8-specific cosmid library. A positive cosmid clone was mapped by fluorescence in situ hybridization to 8p22. The polymerase chain reaction followed by restriction analysis of the PCR product was used to identify allele 2 to be contained in the cosmid clone.

MeSH terms

  • Arylamine N-Acetyltransferase / genetics*
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 8
  • Cloning, Molecular
  • Cosmids
  • DNA Primers / chemistry
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Polymorphism, Restriction Fragment Length


  • DNA Primers
  • Arylamine N-Acetyltransferase
  • NAT2 protein, human