Ultrastructural changes in rat kidney mitochondria in response to the oxygen- or calcium-paradox

Comp Biochem Physiol Comp Physiol. 1994 Feb;107(2):369-74. doi: 10.1016/0300-9629(94)90394-8.

Abstract

Isolated, perfused rat kidney released lactate dehydrogenase in response to the protocol of a standard oxygen-paradox or calcium-paradox. The results are comparable to those found with rat heart. The mitochondria of the proximal tubule cells at the end of the calcium-paradox were swollen, had mitochondrial bars and were subdivided with internal septa; after the oxygen-paradox they exhibited, in addition, many myelin figures. Such ultrastructural changes were characteristic of the mitochondria of skeletal and cardiac muscles damaged by a rise in [Ca2+]i. It is concluded (i) that typical calcium- and oxygen-paradoxes can be produced in kidney, (ii) that these are associated with a rise in [Ca2+]i and (iii) the proximal tubules, in particular, are damaged.

MeSH terms

  • Animals
  • Calcium / metabolism*
  • In Vitro Techniques
  • Kidney Tubules, Proximal / enzymology
  • Kidney Tubules, Proximal / metabolism*
  • Kidney Tubules, Proximal / ultrastructure
  • L-Lactate Dehydrogenase / metabolism
  • Mitochondria / enzymology
  • Mitochondria / metabolism*
  • Mitochondria / ultrastructure
  • Oxygen / metabolism*
  • Rats
  • Rats, Wistar
  • Reperfusion Injury / metabolism*

Substances

  • L-Lactate Dehydrogenase
  • Oxygen
  • Calcium