Detection of HIV-1 DNA in leukocytes using a commercially available assay

Vox Sang. 1994;66(1):78-80. doi: 10.1111/j.1423-0410.1994.tb00282.x.


Recently, an assay for detection of proviral HIV-1 DNA in leukocytes became commercially available. This assay (Amplicor HIV-1 test, Roche Diagnostic Systems) multiplies HIV-1DNA up to a detectable level, using the polymerase chain reaction. We studied performance of this assay on 74 samples from HIV-1-infected patients and on 41 samples from healthy blood donors. Twice a negative control sample appeared to be erroneously reactive. However, sensitivity and specificity on the patient and donor samples both were 100%. To avoid false-positive results, we advise to repeat initially reactive samples if no other data confirm HIV-infection.

MeSH terms

  • Blood Donors
  • CD4-Positive T-Lymphocytes / microbiology
  • DNA, Viral / analysis*
  • False Positive Reactions
  • HIV Infections / blood
  • HIV Infections / diagnosis*
  • HIV Infections / microbiology
  • HIV-1 / isolation & purification*
  • Humans
  • Leukocyte Count
  • Leukocytes / chemistry
  • Leukocytes / microbiology*
  • Polymerase Chain Reaction*
  • Proviruses / isolation & purification*
  • Reagent Kits, Diagnostic*
  • Sensitivity and Specificity


  • DNA, Viral
  • Reagent Kits, Diagnostic