Cellular subsets and epithelial ICAM-1 and HLA-DR expression in human papillomavirus infection of the vulva

Acta Derm Venereol. 1994 Jan;74(1):45-50. doi: 10.2340/00015555744550.

Abstract

Cryostat sections of 20 clinical condylomata of the vulva induced by human papillomavirus and 5 normal control biopsies were examined using immunohistochemistry. The results indicated that in vulvar papillomavirus infection the intraepithelial Langerhans' cells showed abnormal morphology and a significantly lower density than controls. CD1a positive Langerhans' cells were also observed in dermis of condylomata, suggesting an abnormal epithelial traffic of dendritic cells. T lymphocytes with a mean CD4/CD8 ratio of 0.25 and a mean density of 267 +/- 59 cells/mm2 of epithelial section were the main cellular infiltrate in vulvar papillomavirus infection. Most of the T cells were HLA-DR negative. Those condylomata with moderate to severe mononuclear infiltrate showed leucocyte function antigen 1 positive T cells forming small clusters in the lower epithelial half around the ICAM-1 positive keratinocytes. Vulvar warts also showed epithelial areas with overlapped ICAM-1 and HLA-DR expression. Scattered T gamma-delta and B lymphocytes, macrophages and NK cells were observed among the cells of the dermal infiltrate of vulvar condylomata.

MeSH terms

  • Adult
  • Antigens, CD / biosynthesis
  • CD4-CD8 Ratio
  • Cell Adhesion Molecules / biosynthesis*
  • Condylomata Acuminata / immunology
  • Condylomata Acuminata / metabolism*
  • Condylomata Acuminata / pathology
  • Condylomata Acuminata / virology
  • Epithelium / immunology
  • Epithelium / metabolism
  • Epithelium / pathology
  • Female
  • Gene Expression
  • HLA-DR Antigens / biosynthesis*
  • Humans
  • Immunohistochemistry
  • Intercellular Adhesion Molecule-1
  • Killer Cells, Natural / metabolism
  • Killer Cells, Natural / pathology
  • Langerhans Cells / metabolism
  • Langerhans Cells / pathology
  • Lymphocyte Function-Associated Antigen-1 / metabolism
  • Macrophages / metabolism
  • Macrophages / pathology
  • Middle Aged
  • Papillomaviridae*
  • Papillomavirus Infections / immunology
  • Papillomavirus Infections / metabolism*
  • Papillomavirus Infections / pathology
  • Papillomavirus Infections / virology
  • T-Lymphocyte Subsets / metabolism
  • T-Lymphocyte Subsets / pathology
  • Vulvar Diseases / immunology
  • Vulvar Diseases / metabolism*
  • Vulvar Diseases / pathology
  • Vulvar Diseases / virology

Substances

  • Antigens, CD
  • Cell Adhesion Molecules
  • HLA-DR Antigens
  • Lymphocyte Function-Associated Antigen-1
  • Intercellular Adhesion Molecule-1