Major histocompatibility complex-restricted recognition of autologous chronic lymphocytic leukemia by tumor-specific T cells

Immunol Res. 1993;12(4):338-48. doi: 10.1007/BF02935507.


From the peripheral blood of a patient with chronic lymphocytic leukemia (CLL) we generated a T-cell line and clones which recognized autologous CLL. The line comprised T-cell clones which responded to the CLL as well as to autologous Epstein-Barr virus (EBV)-transformed B cells in an HLA-DR-restricted fashion. In addition, the line comprised clones which were CLL-specific and showed no reactivity against EBV-transformed B cells and against autologous peripheral blood mononuclear cells obtained during remission. The proliferative response of the CLL-specific T-cell clone was inhibited by monoclonal antibodies to HLA-DR11, the major histocompatibility complex (MHC)-restrictive element. These results indicate that the MHC class-II molecule of CLL binds a tumor-specific peptide which is recognized by autologous T cells in an MHC class-II-restricted fashion. Such a peptide may serve as a target for immunotherapy.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Antigens, Neoplasm / immunology*
  • B-Lymphocytes / immunology
  • Base Sequence
  • Blotting, Southern
  • CD4-Positive T-Lymphocytes / immunology
  • Cell Line, Transformed
  • Cytotoxicity Tests, Immunologic
  • Genes, Immunoglobulin / genetics
  • HLA Antigens / immunology*
  • Humans
  • Leukemia, Lymphocytic, Chronic, B-Cell / immunology*
  • Lymphocyte Activation / immunology
  • Male
  • Molecular Sequence Data
  • T-Lymphocytes / immunology*


  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • HLA Antigens