Enhancement of CDP-DG:inositol transferase activity in src- and erbB2-transformed cells

Exp Cell Res. 1994 May;212(1):151-4. doi: 10.1006/excr.1994.1129.

Abstract

Phosphatidylinositol (PI) synthesis was activated in Rous sarcoma virus-infected NIH3T3 or activated erbB2-transformed NIH3T3 cells. The in vitro activity of CDP-DG:inositol transferase prepared from these cells was also higher than that from normal parent NIH3T3 cells, although phospholipase C and PI kinase activities were not significantly different among these cells. A tyrosine kinase inhibitor, erbstatin, inhibited the PI synthesis in cultured cells, suggesting that Src and ErbB2-associated tyrosine kinases are involved in activation of CDP-DG:inositol transferase in these cell lines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Phosphatidylinositol 4-Kinase
  • 3T3 Cells
  • Animals
  • CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase
  • Cell Line, Transformed
  • Cell Transformation, Neoplastic*
  • Genes, src / genetics*
  • Hydroquinones / pharmacology
  • Mice
  • Oncogene Proteins, Viral / genetics*
  • Phosphatidylinositols / biosynthesis
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism
  • Protein-Tyrosine Kinases / antagonists & inhibitors
  • Receptor, ErbB-2
  • Transferases (Other Substituted Phosphate Groups) / metabolism*
  • Type C Phospholipases / metabolism

Substances

  • Hydroquinones
  • Oncogene Proteins, Viral
  • Phosphatidylinositols
  • Phosphotransferases (Alcohol Group Acceptor)
  • 1-Phosphatidylinositol 4-Kinase
  • Protein-Tyrosine Kinases
  • Receptor, ErbB-2
  • Transferases (Other Substituted Phosphate Groups)
  • CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase
  • Type C Phospholipases
  • erbstatin