Serotonin-induced contraction in canine coronary artery and saphenous vein: role of a 5-HT1D-like receptor

Life Sci. 1994;54(22):1671-80. doi: 10.1016/0024-3205(94)00607-5.


The identity of the serotonin (5-HT) receptor(s) that mediate(s) contraction in canine coronary artery and saphenous vein remains controversial. Ring segments of endothelium-denuded coronary artery and helical strips of saphenous vein were suspended in organ chambers for measurement of isometric force. 5-HT, alpha Me-5-HT and sumatriptan contracted both coronary artery and saphenous vein and the non-selective 5-HT receptor antagonist 1-naphthylpiperazine (100nM) blocked 5-HT- and sumatriptan-induced contraction in both tissues. The agonist rank order potency for contraction (5-HT > sumatriptan > alpha Me5-HT > 5-MeOT > 5-MeT) was similar in both tissues and was consistent with that for a 5-HT1D receptor. Oligonucleotide primers specific for the 5-HT1D receptor sequence were designed for use in a polymerase chain reaction (PCR). cDNA derived from total RNA or mRNA from canine tissues was used in the PCR. PCR resulted in the amplification of a 632 base pair sequence in both canine coronary artery and saphenous vein; consistent with that expected for the 5-HT1D receptor. Southern blot analysis, with an oligonucleotide probe internal to the sequence amplified by the PCR primers, confirmed that the sequence amplified by PCR was the 5-HT1D receptor. Thus, the 5-HT1D receptor is expressed in canine coronary artery and saphenous vein and taken together with the pharmacological data, supports the possibility that a 5-HT1D-like receptor mediates contraction in these two tissues.

MeSH terms

  • 5-Methoxytryptamine / pharmacology
  • Adrenergic alpha-Antagonists / pharmacology
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Coronary Vessels / drug effects
  • Coronary Vessels / physiology*
  • DNA Primers / chemistry
  • Dioxanes / pharmacology
  • Dogs
  • Dose-Response Relationship, Drug
  • Gene Expression
  • Idazoxan
  • Molecular Sequence Data
  • Muscle Contraction / drug effects*
  • Muscle, Smooth, Vascular / drug effects
  • Muscle, Smooth, Vascular / physiology*
  • Piperazines / pharmacology
  • Polymerase Chain Reaction
  • Prazosin / pharmacology
  • RNA, Messenger / analysis
  • Receptors, Serotonin / genetics
  • Receptors, Serotonin / physiology*
  • Saphenous Vein / drug effects
  • Saphenous Vein / physiology*
  • Serotonin / analogs & derivatives
  • Serotonin / pharmacology
  • Serotonin / physiology
  • Serotonin Receptor Agonists / pharmacology
  • Sumatriptan / pharmacology
  • Tryptamines / pharmacology


  • Adrenergic alpha-Antagonists
  • DNA Primers
  • Dioxanes
  • Piperazines
  • RNA, Messenger
  • Receptors, Serotonin
  • Serotonin Receptor Agonists
  • Tryptamines
  • 5-methyltryptamine
  • alpha-methylserotonin
  • Serotonin
  • 5-Methoxytryptamine
  • 1-(1-naphthyl)piperazine
  • Sumatriptan
  • 5-carboxamidotryptamine
  • Prazosin
  • Idazoxan