Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro

Nature. 1994 May 19;369(6477):207-12. doi: 10.1038/369207a0.


Complete enzymatic replication of DNA from the simian virus 40 origin has been reconstituted with T antigen and highly purified cellular proteins. DNA polymerase-alpha/primase functions primarily to synthesize RNA-DNA primers for initiation of DNA replication at the origin and for priming each Okazaki fragment. A polymerase switching mechanism requiring replication factor C and the proliferating cell nuclear antigen allows two molecules of DNA polymerase-delta to replicate both strands of the double helix conjointly.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cattle
  • DNA Replication*
  • DNA, Viral / biosynthesis*
  • DNA, Viral / genetics
  • DNA-Binding Proteins / metabolism
  • DNA-Directed DNA Polymerase / metabolism
  • Homeodomain Proteins*
  • Humans
  • Minor Histocompatibility Antigens
  • Nuclear Proteins / metabolism
  • Proliferating Cell Nuclear Antigen
  • Proto-Oncogene Proteins c-bcl-2*
  • Replication Protein A
  • Replication Protein C
  • Repressor Proteins*
  • Saccharomyces cerevisiae Proteins*
  • Simian virus 40 / genetics*
  • Templates, Genetic
  • Viral Proteins / metabolism


  • BCL2-related protein A1
  • DNA, Viral
  • DNA-Binding Proteins
  • Homeodomain Proteins
  • MATA1 protein, S cerevisiae
  • Minor Histocompatibility Antigens
  • Nuclear Proteins
  • Proliferating Cell Nuclear Antigen
  • Proto-Oncogene Proteins c-bcl-2
  • RPA1 protein, human
  • Replication Protein A
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Viral Proteins
  • DNA-Directed DNA Polymerase
  • Replication Protein C