Prostatic invasive adenocarcinoma. Effect of combination endocrine therapy (LHRH agonist and flutamide) on the expression and location of proliferating cell nuclear antigen (PCNA)

Pathol Res Pract. 1993 Dec;189(10):1154-60. doi: 10.1016/S0344-0338(11)80838-1.


Expression and location of Proliferating Cell Nuclear Antigen immunostaining in epithelial nuclei were assessed on histological sections from 32 cases of invasive adenocarcinoma of the prostate gland: 20 untreated and 12 treated with combination endocrine therapy or CET. The PCNA-positive nuclei showed homogeneous or granular types of staining or a mixture of both, and a gradation in the intensity of staining. Nuclei with homogeneous patterns appeared darker brown than the lighter granular and mixed patterns. Darker nuclei were more frequently noted, mainly among the epithelial cells adjacent to the stroma, in the untreated cases. In contrast, nuclei with pyknotic chromatin, unstained and corresponding to apoptotic bodies, were more frequently seen in the treated patients. For the untreated invasive adenocarcinomas, the mean proportion of PCNA-stained epithelial nuclei in the 10 cases with an acinar pattern (small and large) was 8.86% (SE 0.23%). The mean value in the 5 cases with a cribriform pattern was 11.76% (SE 0.52%), that is, greater than in the acinar pattern, and decreased from the nuclei in the basal position, or adjacent to the stroma, toward the lumen: 14.40% (SE 0.61%) in the basal position, 11.84% (SE 1.30%) in the intermediate and 9.26% (SE 0.66%) in the lumenal. In the 5 cases with a solid/trabecular pattern, the proportion of PCNA-positive nuclei was 15.74% (SE 2.30%), that is, higher than in all the other patterns, and decreased from the cell layer adjacent to the stroma (17.60%, SE 2.92%) toward the other layers (13.88%, SE 1.71%).(ABSTRACT TRUNCATED AT 250 WORDS)

MeSH terms

  • Adenocarcinoma / chemistry
  • Adenocarcinoma / drug therapy*
  • Adenocarcinoma / pathology*
  • Antineoplastic Combined Chemotherapy Protocols / pharmacology*
  • Flutamide / pharmacology
  • Gonadotropin-Releasing Hormone / analogs & derivatives
  • Gonadotropin-Releasing Hormone / pharmacology
  • Humans
  • Male
  • Nuclear Proteins / biosynthesis
  • Nuclear Proteins / drug effects*
  • Proliferating Cell Nuclear Antigen
  • Prostatic Neoplasms / chemistry
  • Prostatic Neoplasms / drug therapy*
  • Prostatic Neoplasms / pathology*


  • Nuclear Proteins
  • Proliferating Cell Nuclear Antigen
  • Gonadotropin-Releasing Hormone
  • Flutamide