HIV-1/HIV-2 seronegativity in HIV-1 subtype O infected patients

Lancet. 1994 Jun 4;343(8910):1393-4. doi: 10.1016/s0140-6736(94)92524-0.


Nine patients with atypical HIV-1 western blot profiles were diagnosed as having HIV-1 subtype O infection. All the patients were living in France; eight originated from Cameroon and one from France. Lymphocyte DNA amplification by the polymerase chain reaction was only positive when HIV-1 subtype O specific primers were used. Preliminary sequence analysis of amplified products and serological reactivity against a specific subtype O synthetic env peptide confirmed HIV-1 subtype O infection. HIV-1/HIV-2 enzyme-linked immunosorbent assays, especially those based on env peptides or on the sandwich format, can be negative in HIV-1 subtype O infection.

MeSH terms

  • Acquired Immunodeficiency Syndrome / diagnosis
  • Acquired Immunodeficiency Syndrome / microbiology*
  • Adult
  • Blotting, Western
  • Cameroon
  • Enzyme-Linked Immunosorbent Assay
  • France
  • Gene Amplification
  • HIV Seronegativity*
  • HIV-1 / classification*
  • HIV-1 / genetics
  • HIV-1 / immunology
  • HIV-2 / classification*
  • HIV-2 / genetics
  • HIV-2 / immunology
  • Humans
  • Middle Aged
  • Polymerase Chain Reaction