The pili of Pseudomonas aeruginosa strains PAK and PAO bind specifically to the carbohydrate sequence beta GalNAc(1-4)beta Gal found in glycosphingolipids asialo-GM1 and asialo-GM2

Mol Microbiol. 1994 Feb;11(4):715-23. doi: 10.1111/j.1365-2958.1994.tb00349.x.


Pseudomonas aeruginosa employs pili to mediate adherence to epithelial cell surfaces. The pilus adhesin of P. aeruginosa strains PAK and PAO has been shown to bind to the glycolipid asialo-GM1 (Lee et al., 1994--accompanying article). PAK and PAO pili were examined for their abilities to bind to the synthetic beta GalNAc(1-4)beta Gal (a minimal structural carbohydrate receptor sequence of asialo-GM1 and asialo-GM2 proposed by Krivan et al., 1988a) using solid-phase binding assays. Both pili specifically bound to beta GalNAc(1-4)beta Gal. The binding of beta GalNAc(1-4)beta Gal-Biotin to the immobilized PAK and PAO pili was inhibited by corresponding free pili. The receptor binding domain of the PAK pilus resides in the C-terminal disulphide-looped region (residues 128-144) of the pilin structural subunit (Irvin et al., 1989). Biotinylated synthetic peptides corresponding the C-terminal residues 128-144 of P. aeruginosa PAK and PAO pilin molecules were shown to bind to the beta GalNAc(1-4)beta Gal-(bovine serum albumin (BSA)). The binding of biotinylated peptides to beta GalNAc(1-4)beta GAL-BSA was inhibited by PAK pili, Ac-KCTSDQDEQFIPKGCSK-OH (AcPAK(128-144)ox-OH) and Ac-ACKSTQDPMFTPKGCDN-OH (AcPAO(128-144)ox-OH) peptides. (In these peptides Ac denotes N alpha-acetylation of the N-terminus, -OH means a peptide with a free alpha-carboxyl group at the C-terminus and the 'ox' denotes the oxidation of the sulphhydryl groups of Cys-129 and Cys-142.) Both acetylated peptides were also able to inhibit the binding of beta GalNAc(1-4)beta Gal-biotin to the corresponding BSA-Peptide(128-144)ox-OH conjugates.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Bacterial*
  • Amino Acid Sequence
  • Bacterial Adhesion*
  • Bacterial Outer Membrane Proteins / metabolism*
  • Bacterial Proteins / metabolism*
  • Binding, Competitive
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Fimbriae Proteins
  • Fimbriae, Bacterial / metabolism*
  • G(M1) Ganglioside / metabolism*
  • Gangliosides
  • Glycosphingolipids / metabolism*
  • Lectins*
  • Molecular Sequence Data
  • Peptide Fragments / chemical synthesis
  • Peptide Fragments / metabolism
  • Pseudomonas aeruginosa / metabolism*
  • Pseudomonas aeruginosa / ultrastructure
  • Receptors, Cell Surface / metabolism


  • Adhesins, Bacterial
  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Gangliosides
  • Glycosphingolipids
  • Lectins
  • Peptide Fragments
  • Receptors, Cell Surface
  • adhesin, Pseudomonas
  • Fimbriae Proteins
  • ganglio-N-triaosylceramide
  • G(M1) Ganglioside
  • asialo GM1 ganglioside