Enhanced establishment of a virus carrier state in adult CD4+ T-cell-deficient mice

J Virol. 1994 Jul;68(7):4700-4. doi: 10.1128/JVI.68.7.4700-4704.1994.


CD4+ T cells play an important role in regulating the immune response; their contribution to virus clearance is variable. Mice that lack CD4+ T cells (CD4-/- mice) and are therefore unable to produce neutralizing antibodies cleared viscero-lymphotropic lymphocytic choriomeningitis virus (LCMV) strain WE when infected intravenously with a low dose (2 x 10(2) PFU) because of an effective CD8+ cytotoxic T-cell (CTL) response. In contrast, infection with a high dose (2 x 10(6) PFU) of LCMV strain WE led to expansion of antiviral CTL, which disappeared in CD4-/- mice; in contrast, CD4+ T-cell-competent mice developed antiviral memory CTL. This exhaustion of specific CTL caused viral persistence in CD4-/- mice, whereas CD4+ T-cell-competent mice eliminated the virus. After infection of CD4-/- mice with the faster-replicating LCMV strain DOCILE, abrogation of CTL response and establishment of viral persistence developed after infection with a low dose (5 x 10(2) PFU), i.e., an about 100-fold lower dose than in CD(4+)-competent control mice. These results show that absence of T help enhances establishment of an LCMV carrier state in selected situations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral
  • CD4-Positive T-Lymphocytes / immunology*
  • Carrier State*
  • Lymphocytic Choriomeningitis / immunology*
  • Lymphocytic choriomeningitis virus / genetics
  • Lymphocytic choriomeningitis virus / immunology
  • Lymphocytic choriomeningitis virus / isolation & purification
  • Mice
  • Mice, Inbred C57BL
  • Mutation
  • Neutralization Tests
  • T-Lymphocytes, Cytotoxic / immunology


  • Antibodies, Viral