Immunodominant epitopes of glutamic acid decarboxylase 65 and 67 in insulin-dependent diabetes mellitus

Lancet. 1994 Jun 25;343(8913):1607-8. doi: 10.1016/s0140-6736(94)93061-9.


To find the dominant epitopes of a major autoantigen in insulin-dependent diabetes mellitus (IDDM), glutamic acid decarboxylase (GAD), we studied the reactivity of peripheral blood T lymphocytes with peptides covering both major isoforms. A significant response to GAD 65 or GAD 67 peptides was detected in 13 of 15 IDDM patients and in 9 of 10 normal controls. Controls most frequently recognised the central region of GAD 65 (residues 161-243). IDDM patients preferentially recognised residues 473-555 (p < 0.03). T-cell responses to GAD 67 peptides were similar in IDDM patients and controls. T lymphocytes from IDDM patients recognise a distinct dominant epitope of GAD, which may be an important target for the disease process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Autoantigens / immunology
  • Child
  • Child, Preschool
  • Diabetes Mellitus, Type 1 / immunology*
  • Glutamate Decarboxylase / immunology*
  • Humans
  • Immunodominant Epitopes / analysis*
  • Middle Aged
  • T-Lymphocytes / immunology


  • Autoantigens
  • Immunodominant Epitopes
  • Glutamate Decarboxylase