Antibodies inactivating mGluR1 metabotropic glutamate receptor block long-term depression in cultured Purkinje cells

Neuron. 1994 Jun;12(6):1245-55. doi: 10.1016/0896-6273(94)90441-3.


Antibodies were raised against two distinct extracellular sequences of the rat mGluR1 metabotropic glutamate receptor expressed as bacterial fusion proteins. Both antibodies specifically reacted with mGluR1 in the rat cerebellum and inhibited the mGluR1 activity as assessed by the analysis of glutamate-stimulated inositol phosphate formation in CHO cells expressing mGluR1. Using these antibodies, we examined the role of mGluR1 in the induction of long-term depression in cultured Purkinje cells. In voltage-clamped Purkinje cells, current induced by iontophoretically applied glutamate was persistently depressed by depolarization of the Purkinje cells in conjunction with the glutamate application. The mGluR1 antibodies completely blocked the depression of glutamate-induced current. The results indicate that activation of mGluR1 is necessary for the induction of cerebellar long-term depression and that these mGluR1 antibodies can be used as selective antagonists.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Cell Membrane / metabolism
  • Cells, Cultured
  • Cerebellum / physiology*
  • Cricetinae
  • Embryo, Mammalian
  • Excitatory Amino Acid Antagonists
  • Glutamates / pharmacology
  • Glutamic Acid
  • Immunoblotting
  • Immunohistochemistry
  • Inositol Phosphates / metabolism
  • Kinetics
  • Long-Term Potentiation / drug effects
  • Long-Term Potentiation / physiology*
  • Purkinje Cells / drug effects
  • Purkinje Cells / physiology*
  • Rats
  • Receptors, Glutamate / analysis
  • Receptors, Glutamate / physiology*
  • Recombinant Proteins / antagonists & inhibitors
  • Recombinant Proteins / metabolism
  • Time Factors
  • Transfection


  • Excitatory Amino Acid Antagonists
  • Glutamates
  • Inositol Phosphates
  • Receptors, Glutamate
  • Recombinant Proteins
  • Glutamic Acid