Species identification of trichostrongyle nematodes by PCR-linked RFLP

R B Gasser; N B Chilton; H Hoste; L A Stevenson

doi:10.1016/0020-7519(94)90041-8

Species identification of trichostrongyle nematodes by PCR-linked RFLP

Int J Parasitol. 1994 Apr;24(2):291-3. doi: 10.1016/0020-7519(94)90041-8.

Authors

R B Gasser¹, N B Chilton, H Hoste, L A Stevenson

Affiliation

¹ Department of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.

PMID: 7913087
DOI: 10.1016/0020-7519(94)90041-8

Abstract

Restriction fragment length polymorphisms (RFLP) of the second internal transcribed spacer (ITS-2) of ribosomal DNA were examined for 6 species of ruminant nematodes; Trichostrongylus colubriformis, T. axei, T. vitrinus, Haemonchus contortus, Teladorsagia circumcincta and Cooperia oncophora. The ITS-2 of each species was amplified by PCR and restricted with the endonucleases DraI, HinfI, RsaI and VspI. Using this approach, the 6 species could be distinguished. No intraspecific variation in RFLP's has been detected so far in the ITS-2 of these species, suggesting that this technique has considerable potential for the identification of eggs of different nematode species in faecal samples.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
DNA Primers / chemistry
DNA, Ribosomal / analysis*
DNA, Ribosomal / chemistry
Male
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length*
Ruminants / parasitology*
Trichostrongyloidea / genetics
Trichostrongyloidea / isolation & purification*
Trichostrongyloidiasis / parasitology
Trichostrongyloidiasis / veterinary*

Substances

DNA Primers
DNA, Ribosomal