Single and multiple strain colonization in patients with Helicobacter pylori-associated gastritis: detection by macrorestriction DNA analysis

J Infect Dis. 1994 Aug;170(2):473-5. doi: 10.1093/infdis/170.2.473.


Helicobacter pylori isolates from 18 patients were examined by macrorestriction DNA analysis to determine whether > or = 2 strains can coexist in the human stomach. Four biopsy samples from different sites in each patient's stomach were cultured, and 12-14 clones of H. pylori per patient were analyzed. DNA of 15 of the 18 isolates was digestible: 12 by NotI and 3 by NruI. Within 14 of these digestible isolates, the patterns of all clones were identical. The isolate of 1 patient showed two digestion patterns, a finding that was confirmed by arbitrary primer polymerase chain reaction. The typeable isolates of all patients, except 2 who underwent gastroscopy on the same day, differed markedly from each other. These results indicate a noticeable discrepancy between the pronounced interpatient heterogeneity and the rare intrapatient variation of H. pylori.

MeSH terms

  • Base Sequence
  • DNA Primers / chemistry
  • DNA, Bacterial / analysis*
  • Deoxyribonucleases, Type II Site-Specific
  • Electrophoresis, Gel, Pulsed-Field
  • Female
  • Gastritis / microbiology*
  • Helicobacter Infections / microbiology*
  • Helicobacter pylori / classification*
  • Helicobacter pylori / genetics
  • Helicobacter pylori / growth & development
  • Humans
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length


  • DNA Primers
  • DNA, Bacterial
  • endodeoxyribonuclease NruI
  • Deoxyribonucleases, Type II Site-Specific
  • GCGGCCGC-specific type II deoxyribonucleases