Effect of degS-degU mutations on the expression of sigD, encoding an alternative sigma factor, and autolysin operon of Bacillus subtilis

T Tokunaga; M H Rashid; A Kuroda; J Sekiguchi

doi:10.1128/jb.176.16.5177-5180.1994

Effect of degS-degU mutations on the expression of sigD, encoding an alternative sigma factor, and autolysin operon of Bacillus subtilis

J Bacteriol. 1994 Aug;176(16):5177-80. doi: 10.1128/jb.176.16.5177-5180.1994.

Authors

T Tokunaga¹, M H Rashid, A Kuroda, J Sekiguchi

Affiliation

¹ Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.

Abstract

Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy) mutants. The degU32(Hy) mutation caused a 99% reduction in the expression of a sigD-lacZ translational fusion gene constructed in the B. subtilis chromosome. The phosphorylated form of the DegU protein seems to be a regulator for expression of the sigD gene.

MeSH terms

Bacillus subtilis / genetics*
Bacterial Proteins / genetics*
Gene Expression Regulation, Bacterial*
Genes, Bacterial
Genes, Regulator
N-Acetylmuramoyl-L-alanine Amidase / genetics*
Operon
Protein Kinases / genetics
RNA, Messenger / genetics
Sigma Factor / genetics*

Substances

Bacterial Proteins
DegS protein, Bacteria
DegU protein, Bacteria
RNA, Messenger
Sigma Factor
Protein Kinases
N-Acetylmuramoyl-L-alanine Amidase