Abstract
A 5.6 kb DNA fragment from the fungus Phycomyces blakesleeanus has been cloned and sequenced. The fragment contains a gene that probably codes for the enzyme acetyl-coenzyme A synthetase (facA). The amino acid sequence deduced for the P. blakesleeanus protein is highly homologous to those of acetyl-coA-synthetases from other organisms. When placed under the control of a constitutive promoter from Aspergillus nidulans, the cloned gene complemented a facA- mutation of this organism. In P. blakesleeanus, the expression of facA is induced by acetate.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetate-CoA Ligase / biosynthesis
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Acetate-CoA Ligase / genetics*
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Acetates / pharmacology
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Amino Acid Sequence
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Aspergillus nidulans / genetics
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Base Sequence
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Cloning, Molecular
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Enzyme Induction / drug effects
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Gene Expression Regulation, Fungal / drug effects
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Genes, Fungal / genetics*
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Introns / genetics
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Molecular Sequence Data
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Phycomyces / enzymology
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Phycomyces / genetics*
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RNA, Messenger / genetics
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Recombinant Proteins / biosynthesis
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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Acetates
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RNA, Messenger
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Recombinant Proteins
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Acetate-CoA Ligase