Isolation of the facA (acetyl-CoA synthetase) gene of Phycomyces blakesleeanus

Mol Gen Genet. 1994 Aug 2;244(3):278-86. doi: 10.1007/BF00285455.

Abstract

A 5.6 kb DNA fragment from the fungus Phycomyces blakesleeanus has been cloned and sequenced. The fragment contains a gene that probably codes for the enzyme acetyl-coenzyme A synthetase (facA). The amino acid sequence deduced for the P. blakesleeanus protein is highly homologous to those of acetyl-coA-synthetases from other organisms. When placed under the control of a constitutive promoter from Aspergillus nidulans, the cloned gene complemented a facA- mutation of this organism. In P. blakesleeanus, the expression of facA is induced by acetate.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetate-CoA Ligase / biosynthesis
  • Acetate-CoA Ligase / genetics*
  • Acetates / pharmacology
  • Amino Acid Sequence
  • Aspergillus nidulans / genetics
  • Base Sequence
  • Cloning, Molecular
  • Enzyme Induction / drug effects
  • Gene Expression Regulation, Fungal / drug effects
  • Genes, Fungal / genetics*
  • Introns / genetics
  • Molecular Sequence Data
  • Phycomyces / enzymology
  • Phycomyces / genetics*
  • RNA, Messenger / genetics
  • Recombinant Proteins / biosynthesis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • Acetates
  • RNA, Messenger
  • Recombinant Proteins
  • Acetate-CoA Ligase

Associated data

  • GENBANK/M94729