HIV and T cell expansion in splenic white pulps is accompanied by infiltration of HIV-specific cytotoxic T lymphocytes

Cell. 1994 Aug 12;78(3):373-87. doi: 10.1016/0092-8674(94)90417-0.


Human immunodeficiency virus (HIV) replication and T cell proliferation were investigated in situ by a PCR-based analysis of individual microdissected splenic white pulps. Founder effects, revealed by an exquisite compartmentalization of HIV genotypes and T cells, indicated the recruitment of latently infected CD4+ T cells through highly localized antigen presentation rather than the infection of CD4+ T lymphoblasts by blood-borne virus or immune complexes. HIV-infected white pulps could be infiltrated by HIV-specific cytotoxic T lymphocytes, thereby implicating them in CD4+ T cell destruction in vivo. Together these data describe an iterative and deleterious mechanism of antigen-driven T cell recruitment and activation, as well as HIV replication and spread, with consequent destruction of the newly infected cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigen Presentation
  • Base Sequence
  • CD4-Positive T-Lymphocytes / immunology
  • Cell Movement
  • HIV / genetics*
  • HIV / growth & development
  • HIV Seropositivity / immunology*
  • Humans
  • Lymphocyte Activation
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Proviruses / genetics
  • Proviruses / growth & development
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Sequence Homology, Amino Acid
  • Spleen / cytology
  • Spleen / immunology*
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocytes, Cytotoxic / immunology*


  • Receptors, Antigen, T-Cell, alpha-beta