A chimeric homeodomain protein causes self-compatibility and constitutive sexual development in the mushroom Coprinus cinereus

EMBO J. 1994 Sep 1;13(17):4054-9.

Abstract

The A mating type genes of the mushroom Coprinus cinereus encode two classes of putative transcription factor with distinctive homeodomain motifs (HD1 and HD2). A successful mating brings together different allelic forms of these genes and this triggers part of a developmental sequence required for sexual reproduction. In this report we provide evidence that this developmental programme is promoted by a physical interaction between the two classes of homeodomain protein. Rare dominant mutations conferring self-compatibility map to the A locus and result in constitutive operation of the A-regulated developmental pathway. Our molecular analysis of one of these mutations shows that it has generated a chimeric gene by inframe fusion of an HD2 and an HD1 gene. Fusion has overcome the normal incompatibility between two proteins coded by genes of the same A locus and generated a protein that is sufficient to promote development in the absence of any other active A mating type genes. The fusion protein retains most of the HD2 sequence, but only the C-terminal part of the HD1 protein. It has only the HD2 homeodomain motif as a potential DNA binding domain fused to an essential C-terminal region of the HD1 protein, which in a normal HD1-HD2 protein complex may be the major activation domain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Coprinus / cytology
  • Coprinus / genetics*
  • Fungal Proteins / biosynthesis
  • Fungal Proteins / genetics*
  • Gene Rearrangement
  • Genes, Fungal / genetics*
  • Genes, Homeobox / genetics*
  • Genes, Mating Type, Fungal*
  • Homeodomain Proteins / biosynthesis*
  • Homeodomain Proteins / genetics
  • Molecular Sequence Data
  • Mutation
  • Recombinant Fusion Proteins / biosynthesis
  • Reproduction / genetics
  • Sequence Deletion
  • Sex*
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics

Substances

  • Fungal Proteins
  • HD1 protein, Coprinus cinereus
  • HD2 protein, Coprinus cinereus
  • Homeodomain Proteins
  • Recombinant Fusion Proteins
  • Transcription Factors