Expression of antral gastrin and somatostatin mRNA in Helicobacter pylori-infected subjects

Am J Gastroenterol. 1994 Sep;89(9):1515-9.


Objectives: We investigated the gene expression of antral gastrin and somatostatin in Helicobacter pylori-infected subjects. Twelve asymptomatic men with normal endoscopic findings participated in this study, four of whom were infected with H. pylori.

Methods: Biopsy specimens of the gastric mucosa were obtained after subjects had fasted overnight. Serum gastrin levels, antral gastrin and somatostatin content, and antral G- and D-cell densities also were measured.

Results: Fasting serum gastrin levels were significantly higher in H. pylori-positive subjects than in H. pylori-negative subjects. There were no differences between groups in antral gastrin content, G-cell density, or gastrin mRNA levels. Antral somatostatin content, D-cell density, somatostatin mRNA levels, and the somatostatin mRNA:D-cell density ratio were significantly decreased in H. pylori-positive subjects.

Conclusions: Our results suggest that in addition to reduced antral D-cell density, a suppression of somatostatin synthesis in D-cells may have contributed to the decrease in antral somatostatin in H. pylori-infected subjects.

MeSH terms

  • Adult
  • Blotting, Northern
  • Cell Count
  • Gastric Mucosa / metabolism
  • Gastric Mucosa / pathology
  • Gastrins / biosynthesis*
  • Gastrins / genetics
  • Gene Expression
  • Helicobacter Infections / metabolism*
  • Helicobacter pylori / isolation & purification*
  • Humans
  • Male
  • Pyloric Antrum / metabolism
  • Pyloric Antrum / pathology
  • RNA, Messenger / genetics
  • Radioimmunoassay
  • Somatostatin / biosynthesis*
  • Somatostatin / genetics


  • Gastrins
  • RNA, Messenger
  • Somatostatin