Mediation of renal cyst formation by hepatocyte growth factor

Lancet. 1994 Sep 17;344(8925):789-91. doi: 10.1016/s0140-6736(94)92344-2.

Abstract

Hepatocyte growth factor (HGF) is a potent mitogen for renal tubular cells, and HGF and its receptor (c-met proto-oncogene product, Met) can induce lumen formation in epithelial cells. We measured the concentration of HGF in cyst fluids from patients with renal cystic diseases. The concentration of HGF was high in proximal cyst fluid (mean 2.45 vs 0.42 ng/mL in distal cysts). Cyclic AMP concentration was higher in distal than in proximal cysts (663 vs 6.0 pmol/L). mRNA of HGF and Met were co-expressed in cyst walls from cases with polycystic kidney disease. These findings suggest that HGF is a growth factor that may mediate human renal cyst genesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • Creatinine / analysis
  • Creatinine / blood
  • Cyclic AMP / isolation & purification
  • DNA Primers / chemistry
  • DNA, Complementary / chemistry
  • Hepatocyte Growth Factor / analysis*
  • Hepatocyte Growth Factor / genetics
  • Hepatocyte Growth Factor / physiology
  • Humans
  • Molecular Sequence Data
  • Polycystic Kidney, Autosomal Dominant / chemistry*
  • Polycystic Kidney, Autosomal Dominant / pathology
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins / analysis*
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins c-met
  • RNA, Messenger / analysis
  • Radioimmunoassay
  • Receptor Protein-Tyrosine Kinases / analysis*
  • Receptor Protein-Tyrosine Kinases / genetics
  • Sodium / analysis

Substances

  • DNA Primers
  • DNA, Complementary
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Hepatocyte Growth Factor
  • Sodium
  • Creatinine
  • Cyclic AMP
  • Proto-Oncogene Proteins c-met
  • Receptor Protein-Tyrosine Kinases