Sequence of a dihydrofolate reductase-encoding gene from Candida albicans

Gene. 1994 Sep 15;147(1):115-8. doi: 10.1016/0378-1119(94)90049-3.

Abstract

The nucleotide (nt) sequence of the dihydrofolate reductase (DHFR)-encoding gene (DFR1) of Candida albicans was determined. The gene contains an open reading frame of 576 nt, coding for a protein of 192 amino acid (aa) residues (calculated M(r) 22,222), that is 38.5 and 31% similar to the Saccharomyces cerevisiae and human enzymes, respectively. The first 36 residues, at the N terminus, of the deduced aa sequence are identical to those determined by sequencing of the purified enzyme from C. albicans. Putative transcription start points were also determined. Restriction-fragment-length polymorphism analysis of the DFR1 chromosomal region suggests the presence of a single copy of the gene per haploid genome and shows a limited variability among the different C. albicans strains tested.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Candida albicans / genetics*
  • DNA, Fungal / genetics
  • Genes, Fungal*
  • Molecular Sequence Data
  • Polymorphism, Restriction Fragment Length
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Tetrahydrofolate Dehydrogenase / genetics*

Substances

  • DNA, Fungal
  • Tetrahydrofolate Dehydrogenase

Associated data

  • GENBANK/X78968