Cloning and expression of rat prostaglandin endoperoxide synthase (cyclooxygenase)-2 cDNA

Biochem Biophys Res Commun. 1993 Dec 15;197(2):494-500. doi: 10.1006/bbrc.1993.2506.

Abstract

Rat prostaglandin endoperoxidase synthase-2 (PGHS-2) cDNA was cloned from rat calvarial osteoblasts total RNA by RT-PCR. The primary sequence of rat PGHS-2 had 98% and 92% identity to the mouse and human enzymes, respectively. Transfection of the rat PGHS-2 cDNA into COS 7 cells, followed by the addition of 20 microM arachidonic acid, resulted in a dramatic increase in PGE2 released from these cells. The amount of PGE2 produced was comparable to that obtained from cells similarly transfected with human PGHS-1 cDNA. In the rat paw carrageenin-oedema inflammatory model, the injected paw had elevated levels of PGHS-2 mRNA compared to the control paw. In a rat pyrexia model, injection of the pyrogen lipopolysaccharide, resulted in elevated levels of PGHS-2 mRNA in the brain. These results suggest that PGHS-2 expression plays a role both in inflammation and fever.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Carrageenan
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary / metabolism*
  • Dinoprostone / metabolism
  • Edema / chemically induced
  • Edema / enzymology
  • Enzyme Induction
  • Gene Expression
  • Humans
  • Inflammation / enzymology
  • Lipopolysaccharides / pharmacology
  • Male
  • Mice
  • Molecular Sequence Data
  • Osteoblasts / metabolism*
  • Polymerase Chain Reaction / methods
  • Prostaglandin-Endoperoxide Synthases / biosynthesis*
  • Prostaglandin-Endoperoxide Synthases / genetics*
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Homology, Amino Acid
  • Transfection

Substances

  • DNA, Complementary
  • Lipopolysaccharides
  • RNA, Messenger
  • Carrageenan
  • Prostaglandin-Endoperoxide Synthases
  • Dinoprostone

Associated data

  • GENBANK/D17642
  • GENBANK/D17796
  • GENBANK/D17797
  • GENBANK/D17798
  • GENBANK/D17799
  • GENBANK/D17800
  • GENBANK/D17801
  • GENBANK/D17802
  • GENBANK/L25925
  • GENBANK/U04828