Cloning of rat interleukin-13 (IL-13) cDNA and analysis of IL-13 gene expression in experimental glomerulonephritis

Biochem Biophys Res Commun. 1993 Dec 15;197(2):612-8. doi: 10.1006/bbrc.1993.2523.

Abstract

Using a reverse transcriptase-polymerase chain reaction method, a cDNA for the complete coding region and part of the 5'-untranslated region of rat IL-13 was cloned from rat renal cortex RNA following the induction of anti-glomerular basement membrane antibody-induced glomerulonephritis. The coding region of IL-13 cDNA displays 74% and 87% sequence identity with the coding regions of human and mouse IL-13 cDNA, respectively. The deduced amino acid sequence of rat IL-13 reveals 63% and 79% homology with the human and mouse proteins, respectively. Using the rat IL-13 cDNA as a molecular probe, increased production of IL-13 mRNA in renal and splenic tissue is demonstrated in the first 48 hours of antibody-induced glomerulonephritis in the rat. The data suggest a role for IL-13 in the inflammatory response in vivo.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers
  • DNA, Complementary / isolation & purification
  • Gene Expression Regulation*
  • Glomerulonephritis / metabolism*
  • Humans
  • Interleukin-13
  • Interleukins / biosynthesis*
  • Interleukins / genetics*
  • Kidney Glomerulus / metabolism*
  • Male
  • Mice
  • Molecular Sequence Data
  • Oligonucleotides, Antisense
  • Polymerase Chain Reaction
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / isolation & purification
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Homology, Amino Acid

Substances

  • DNA Primers
  • DNA, Complementary
  • Interleukin-13
  • Interleukins
  • Oligonucleotides, Antisense
  • RNA, Messenger

Associated data

  • GENBANK/D17642
  • GENBANK/D17796
  • GENBANK/D17797
  • GENBANK/D17798
  • GENBANK/D17799
  • GENBANK/D17800
  • GENBANK/D17801
  • GENBANK/D17802
  • GENBANK/L26913
  • GENBANK/U04828