Somatic cell hybrids producing antibodies specific for the tumor antigen of simian virus 40

Proc Natl Acad Sci U S A. 1978 May;75(5):2320-3. doi: 10.1073/pnas.75.5.2320.

Abstract

We have produced somatic cell hybrids between mouse myeloma cells deficient in hypoxanthine phosphoribosyltransferase IMP: pyrophosphate phosphoribosyltransferase; EC 2.4.2.8) and spleen cells derived from mice primed with either syngeneic or allogeneic cells transformed by simian virus 40. Such hybrids produced antibodies specific for simian virus 40 tumor (T) antigen. Only four of twelve independent hybrid cell cultures produced antibodies against simian virus 40 T antigen that crossreacted with the T antigen induced by BK virus, a human papovavirus isolated from patients who had undergone immunosuppressive therapy.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Neoplasm / biosynthesis*
  • Antibodies, Viral / biosynthesis*
  • Antibody Specificity*
  • BK Virus / immunology*
  • Clone Cells / immunology
  • Cross Reactions
  • Epitopes
  • Hybrid Cells / immunology*
  • Mice
  • Myeloma Proteins / immunology
  • Polyomavirus / immunology*
  • Simian virus 40 / immunology*
  • Spleen / immunology

Substances

  • Antibodies, Neoplasm
  • Antibodies, Viral
  • Epitopes
  • Myeloma Proteins