Differential expression of glycine receptor subunits in the retina of the rat: a study using immunohistochemistry and in situ hybridization

Vis Neurosci. Jul-Aug 1994;11(4):721-9. doi: 10.1017/s0952523800003023.

Abstract

Immunohistochemistry and in situ hybridization were used to study the distribution of glycine receptor (GlyR) subunits and the GlyR-associated protein gephyrin in the rat retina. Monoclonal antibodies against the alpha and beta subunits of the GlyR and gephyrin showed a strong punctate labeling pattern in the inner plexiform layer. Glycine receptor mRNAs were found in the inner nuclear layer and the ganglion cell layer. The alpha 1 subunit mRNA is predominantly present in the outer half of the INL and on some but not all ganglion cells. GlyR alpha 2 subunit mRNA is predominantly present in the inner half of the INL and on nearly all cells in the ganglion cell layer. GlyR alpha 3-, GlyR beta-, and gephyrin-mRNAs are present in the entire INL and in cells in the ganglion cell layer. The differential expression of glycine receptor subunits indicates a functional diversity of glycine receptors in the retina.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / analysis
  • Fluorescent Antibody Technique
  • Gene Expression
  • In Situ Hybridization
  • Interneurons / chemistry
  • Membrane Proteins / analysis
  • RNA, Messenger / analysis
  • Rats
  • Receptors, Glycine / analysis*
  • Retina / chemistry*
  • Retinal Ganglion Cells / chemistry

Substances

  • Carrier Proteins
  • Membrane Proteins
  • RNA, Messenger
  • Receptors, Glycine
  • gephyrin