Growth dynamics of pancreatic islet cell populations during fetal and neonatal development of the rat

Dev Dyn. 1994 Jun;200(2):163-75. doi: 10.1002/aja.1002000208.

Abstract

Rats from 18 days fetal to 28 days neonatal ages were studied for the total population sizes and cell proliferation activities of insulin secreting B cells, glucagon secreting A cells, somatostatin secreting D cells, and pancreatic polypeptide secreting PP cells. Cell population sizes were assessed by morphometric quantitation of immunohistochemically stained cells by a linear scanning method and cell proliferation activities were estimated by [3H]-thymidine labeling indices of these cell populations. There was a continuous increase in population sizes for all 4 islet cell types, with the fastest increase occurring in the last 4 days of gestation. The accelerated growth of these islet cell populations during late gestation was accomplished by a high cell proliferative activity at 20-22 days of gestation and a large influx of undifferentiated epithelial cells differentiating into the specific islet cell populations during this period. There was a reduction of population growth and cell proliferation for all islet cell types during the first 3-4 days of life. Growth activities continued for all islet cell populations after the 4th postnatal day, with a renewed acceleration in growth activities for B and A cells at this time. After the 10th neonatal day, the cell proliferation and total population growth continued at slow rates for all 4 islet cell types. The contribution from undifferentiated epithelial cells into the specific islet cell populations was negligible for B and A cells but continued at a low rate for PP and D cells during the first 10 days after birth. For B and A cell populations, there was a possibility that some cell loss occurred during the first 10 days of neonatal life. These dynamic changes of the growth characteristics provide a basis for understanding the abnormal growth of the endocrine pancreas.

MeSH terms

  • Animals
  • Animals, Newborn
  • Cell Count
  • Cell Death
  • Cell Differentiation
  • Cell Division
  • Cell Size
  • Embryonic and Fetal Development / physiology
  • Epithelial Cells
  • Female
  • Islets of Langerhans / cytology*
  • Islets of Langerhans / embryology*
  • Male
  • Morphogenesis
  • Pancreas / cytology
  • Pregnancy
  • Rats
  • Rats, Sprague-Dawley