Ethnic distribution of slow acetylator mutations in the polymorphic N-acetyltransferase (NAT2) gene

Pharmacogenetics. 1994 Jun;4(3):125-34. doi: 10.1097/00008571-199406000-00003.

Abstract

The acetylation polymorphism may affect rates of activation or detoxification of common carcinogens, thereby influencing cancer risk. Our aim was to define the ethnic distribution of the major slow acetylator mutations in the polymorphic N-acetyltransferase gene, in order to provide background data for epidemiological studies. Our results contain new analyses on 803 individuals, including 365 new specimens and 438 specimens that had been partly characterized in an earlier study. Tests were done to establish the specificity and reproducibility (98%) of our PCR assays. The recognized slow acetylator mutations, 191A, 481T, 590A, and 857A (which correspond to alleles M4 and M4b; M1 and r3; M2/r2; and M3 and S3, respectively), accounted for nearly all slow acetylator alleles among blacks, whites, Asian Indians, Hispanics, Koreans, Japanese, Hong Kong Chinese, Taiwanese, Filipinos and Samoans. The ethnic distribution supports an interpretation that the acetylation polymorphism existed before Paleolithic splitting of human populations from Africa. We identified two additional NAT2 mutations, suggesting that other rare alleles are likely to be found.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Adolescent
  • Adult
  • Alleles
  • Arylamine N-Acetyltransferase / genetics*
  • Base Sequence
  • Biological Evolution
  • Continental Population Groups / genetics
  • DNA Primers / genetics
  • Female
  • Gene Frequency
  • Humans
  • Infant, Newborn
  • Middle Aged
  • Molecular Sequence Data
  • Mutation*
  • Polymerase Chain Reaction / statistics & numerical data
  • Polymorphism, Genetic*
  • Pregnancy
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • DNA Primers
  • Arylamine N-Acetyltransferase
  • NAT2 protein, human