Detection of autoantibodies to the 90 kDa heat shock protein in systemic lupus erythematosus and other autoimmune diseases

Br J Rheumatol. 1994 Oct;33(10):923-6. doi: 10.1093/rheumatology/33.10.923.


Expression of the highly conserved 90 kDa heat shock protein (Hsp90) is elevated in the peripheral blood mononuclear cells of approximately 25% of patients with SLE. Conflicting data have been published about the frequency of antibodies to Hsp90 with the previous methodology using a complex Western blot system. We now describe an ELISA to measure autoantibodies to Hsp90 and Hsp70 in SLE patients, healthy controls and patients with a variety of autoimmune rheumatic diseases. IgG and IgM antibodies were elevated in 26 and 35% of SLE patients, respectively. These results show autoantibodies to Hsp90 (but not Hsp70) are elevated in a significant proportion of patients with SLE (P < 0.025) compared to healthy controls; and that those with raised antibody levels were more likely to have renal disease and a low C3 level (P < 0.02).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthritis, Rheumatoid / blood
  • Arthritis, Rheumatoid / immunology
  • Autoantibodies / blood*
  • Autoimmune Diseases / blood
  • Autoimmune Diseases / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • HLA Antigens / blood
  • HSP70 Heat-Shock Proteins / immunology
  • HSP90 Heat-Shock Proteins / immunology*
  • Humans
  • Immunoglobulin G / blood*
  • Immunoglobulin M / blood*
  • Lupus Erythematosus, Systemic / blood
  • Lupus Erythematosus, Systemic / immunology*
  • Male
  • Scleroderma, Systemic / blood
  • Scleroderma, Systemic / immunology


  • Autoantibodies
  • HLA Antigens
  • HSP70 Heat-Shock Proteins
  • HSP90 Heat-Shock Proteins
  • Immunoglobulin G
  • Immunoglobulin M