Adenoviral vectors as functional retrograde neuronal tracers

Brain Res. 1994 Jun 13;648(1):171-5. doi: 10.1016/0006-8993(94)91919-4.


Adenoviruses have been recently recognized as a highly efficient system for gene delivery to various tissues. The ability of replication-defective recombinant adenovirus to transfer the lacZ reporter gene encoding beta-galactosidase to nerve cells in various brain structures has been demonstrated. Here, on the continuation of these studies, we present evidence that the adenovirus can be transported in a retrograde manner to nerve cell bodies from axonal terminals. This method may be of great value for infecting selected subsets of specific neurons for either anatomo-functional studies or even therapeutic purposes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Avian Sarcoma Viruses / genetics
  • Avian Sarcoma Viruses / physiology*
  • Escherichia coli / enzymology
  • Genetic Vectors*
  • Male
  • Neostriatum / enzymology
  • Neostriatum / metabolism
  • Neurons / physiology*
  • Rats
  • Rats, Wistar
  • Repetitive Sequences, Nucleic Acid
  • Tyrosine 3-Monooxygenase / immunology
  • Tyrosine 3-Monooxygenase / metabolism
  • beta-Galactosidase / metabolism


  • Tyrosine 3-Monooxygenase
  • beta-Galactosidase