Loss of heterozygosity of chromosome 9p21 is associated with the immortal phenotype of neoplastic human head and neck keratinocytes

O Loughran; K G Edington; I J Berry; L J Clark; E K Parkinson

Loss of heterozygosity of chromosome 9p21 is associated with the immortal phenotype of neoplastic human head and neck keratinocytes

Cancer Res. 1994 Oct 1;54(19):5045-9.

Authors

O Loughran¹, K G Edington, I J Berry, L J Clark, E K Parkinson

Affiliation

¹ CRC Beatson Laboratories, Bearsden, Glasgow, Scotland.

PMID: 7923114

Abstract

Human chromosomes 1,4,6, and 9 harbor genes which induce cellular senescence in vitro but a role for their inactivation in human tumors is not established. To investigate this we searched for loss of heterozygosity (LOH) on these chromosomes in keratinocyte cultures obtained from different stages of human squamous cell carcinoma progression. There was consistent LOH between markers D9S171 and D9S157 in 9 of 9 (100%) informative immortal cultures and in one line which entered crisis, but 0 of 7 informative senescent cultures showed LOH. These results suggest that inactivation of a gene at 9p21 is important but insufficient for human squamous cell carcinoma keratinocyte immortalization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Calcium-Binding Proteins / genetics
Calcium-Binding Proteins / physiology
Carcinoma, Squamous Cell / genetics*
Cells, Cultured
Chromosome Deletion*
Chromosomes, Human, Pair 9*
Head and Neck Neoplasms / genetics*
Humans
Keratinocytes / ultrastructure*
Phenotype
S100 Calcium-Binding Protein A4
S100 Proteins*

Substances

Calcium-Binding Proteins
S100 Calcium-Binding Protein A4
S100 Proteins
S100A4 protein, human