A Novel Kinase Cascade Triggered by Stress and Heat Shock That Stimulates MAPKAP kinase-2 and Phosphorylation of the Small Heat Shock Proteins

Cell. 1994 Sep 23;78(6):1027-37. doi: 10.1016/0092-8674(94)90277-1.

Abstract

MAPK-activated protein kinase-2 (MAPKAP kinase-2) is activated in vitro by the p42 and p44 isoforms of MAPK (p42/p44MAPK). In several cell lines, however, MAPKAP kinase-2 is activated by sodium arsenite, heat shock, or osmotic stress and not by agonists that activate p42/p44MAPK. We have identified a MAPK-like enzyme that acts as a MAPKAP kinase-2 reactivating kinase (RK). RK is recognized by an antiserum raised against a Xenopus MAPK (Mpk2), which is most similar to HOG1 from S. cerevisiae. We also identified a RK kinase (RKK) on the basis of its ability to activate either RK or a GST-Mpk2 fusion protein. The RKK, RK, and MAPKAP kinase-2 constitute a new stress-activated signal transduction pathway in vertebrates that is distinct from the classical MAPK cascade.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Arsenites / pharmacology
  • Base Sequence
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • HSP27 Heat-Shock Proteins
  • Heat-Shock Proteins / metabolism*
  • Hot Temperature / adverse effects
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Mitogen-Activated Protein Kinases*
  • Molecular Sequence Data
  • Neoplasm Proteins / metabolism
  • Phosphorylation
  • Protein Kinases*
  • Protein-Serine-Threonine Kinases / metabolism*
  • Rats
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Signal Transduction*
  • Xenopus / genetics
  • Xenopus Proteins*

Substances

  • Arsenites
  • HSP27 Heat-Shock Proteins
  • HSPB1 protein, human
  • Heat-Shock Proteins
  • Hspb1 protein, rat
  • Intracellular Signaling Peptides and Proteins
  • Neoplasm Proteins
  • Saccharomyces cerevisiae Proteins
  • Xenopus Proteins
  • Protein Kinases
  • MAP-kinase-activated kinase 2
  • Mpk2 protein, Xenopus
  • Protein-Serine-Threonine Kinases
  • Calcium-Calmodulin-Dependent Protein Kinases
  • HOG1 protein, S cerevisiae
  • Mitogen-Activated Protein Kinases
  • mapk14a protein, Xenopus
  • arsenite

Associated data

  • GENBANK/X80751