Abstract
In plants, resistance to a pathogen is frequently correlated with a genetically defined interaction between a plant resistance gene and a corresponding pathogen avirulence gene. A simple model explains these gene-for-gene interactions: avirulence gene products generate signals (ligands), and resistance genes encode cognate receptors. The A. thaliana RPS2 gene confers resistance to the bacterial pathogen P. syringae carrying the avirulence gene avrRpt2. A map-based positional cloning strategy was used to identify RPS2. The identification of RPS2 was verified using a newly developed transient assay for RPS2 function and by genetic complementation in transgenic plants. RPS2 encodes a novel 105 kDa protein containing a leucine zipper, a nucleotide-binding site, and 14 imperfect leucine-rich repeats.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Arabidopsis / genetics*
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Arabidopsis / microbiology
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Arabidopsis Proteins*
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Base Sequence
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Binding Sites
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Chromosome Walking
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Cloning, Molecular
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Genes, Plant / genetics*
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Genetic Complementation Test
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Genetic Vectors
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Immunity, Innate / genetics
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Leucine Zippers
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Molecular Sequence Data
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Multigene Family / genetics
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Mutation
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Nucleotides / metabolism
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Plant Diseases / genetics*
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Plant Proteins / genetics*
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Plants, Genetically Modified
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Pseudomonas Infections*
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Repetitive Sequences, Nucleic Acid
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Sequence Homology, Amino Acid
Substances
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Arabidopsis Proteins
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Nucleotides
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Plant Proteins
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RPS2 protein, Arabidopsis
Associated data
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GENBANK/U12856
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GENBANK/U12857
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GENBANK/U12858
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GENBANK/U12859
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GENBANK/U12860