The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats

Cell. 1994 Sep 23;78(6):1089-99. doi: 10.1016/0092-8674(94)90282-8.


In plants, resistance to a pathogen is frequently correlated with a genetically defined interaction between a plant resistance gene and a corresponding pathogen avirulence gene. A simple model explains these gene-for-gene interactions: avirulence gene products generate signals (ligands), and resistance genes encode cognate receptors. The A. thaliana RPS2 gene confers resistance to the bacterial pathogen P. syringae carrying the avirulence gene avrRpt2. A map-based positional cloning strategy was used to identify RPS2. The identification of RPS2 was verified using a newly developed transient assay for RPS2 function and by genetic complementation in transgenic plants. RPS2 encodes a novel 105 kDa protein containing a leucine zipper, a nucleotide-binding site, and 14 imperfect leucine-rich repeats.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis / microbiology
  • Arabidopsis Proteins*
  • Base Sequence
  • Binding Sites
  • Chromosome Walking
  • Cloning, Molecular
  • Genes, Plant / genetics*
  • Genetic Complementation Test
  • Genetic Vectors
  • Immunity, Innate / genetics
  • Leucine Zippers
  • Molecular Sequence Data
  • Multigene Family / genetics
  • Mutation
  • Nucleotides / metabolism
  • Plant Diseases / genetics*
  • Plant Proteins / genetics*
  • Plants, Genetically Modified
  • Pseudomonas Infections*
  • Repetitive Sequences, Nucleic Acid
  • Sequence Homology, Amino Acid


  • Arabidopsis Proteins
  • Nucleotides
  • Plant Proteins
  • RPS2 protein, Arabidopsis

Associated data

  • GENBANK/U12856
  • GENBANK/U12857
  • GENBANK/U12858
  • GENBANK/U12859
  • GENBANK/U12860