Involvement of growth factor receptors in the mammalian UVC response

Cell. 1994 Sep 23;78(6):963-72. doi: 10.1016/0092-8674(94)90272-0.

Abstract

Irradiation of HeLa cells with short-wavelength ultraviolet light (UVC) induces the modification and activation of the preexisting transcription factors c-Fos-c-Jun (AP-1) and TCF/Elk-1, as well as the protein synthesis independent transcriptional activation of the c-fos and c-jun genes. This response to UVC is mediated via obligatory cytoplasmic signal transduction, involving Ras and Raf, Src, and MAP kinases. The UVC response is inhibited by prior down-modulation of growth factor receptor signaling upon growth factor prestimulation, by suramin (an inhibitor of receptor activation) or by expression of a dominant negative epidermal growth factor (EGF) receptor mutant. These data suggest the involvement of several growth factor receptors in the UVC response. Indeed, UVC induces the suramin-inhibitable immediate tyrosine phosphorylation of the EGF receptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • Cell Nucleus / physiology
  • DNA-Binding Proteins*
  • ErbB Receptors / genetics
  • ErbB Receptors / metabolism
  • Gene Expression Regulation
  • Genes, fos / genetics
  • HeLa Cells
  • Humans
  • Phosphorylation
  • Promoter Regions, Genetic / genetics
  • Protein-Serine-Threonine Kinases / metabolism
  • Protein-Tyrosine Kinases / metabolism
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins c-fos / biosynthesis
  • Proto-Oncogene Proteins c-fos / genetics
  • Proto-Oncogene Proteins c-raf
  • Receptors, Growth Factor / antagonists & inhibitors
  • Receptors, Growth Factor / genetics
  • Receptors, Growth Factor / metabolism*
  • Retroviridae Proteins, Oncogenic / metabolism
  • Signal Transduction / radiation effects*
  • Suramin / pharmacology
  • Transcription Factor AP-1 / metabolism
  • Transcription Factors / metabolism
  • Transcription, Genetic
  • Tyrosine / metabolism
  • Ultraviolet Rays*
  • ets-Domain Protein Elk-1

Substances

  • DNA-Binding Proteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-fos
  • Receptors, Growth Factor
  • Retroviridae Proteins, Oncogenic
  • Transcription Factor AP-1
  • Transcription Factors
  • ets-Domain Protein Elk-1
  • Tyrosine
  • Suramin
  • ErbB Receptors
  • Protein-Tyrosine Kinases
  • Protein-Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-raf
  • Calcium-Calmodulin-Dependent Protein Kinases