Human TAFII30 is present in a distinct TFIID complex and is required for transcriptional activation by the estrogen receptor

X Jacq; C Brou; Y Lutz; I Davidson; P Chambon; L Tora

doi:10.1016/0092-8674(94)90404-9

Human TAFII30 is present in a distinct TFIID complex and is required for transcriptional activation by the estrogen receptor

Cell. 1994 Oct 7;79(1):107-17. doi: 10.1016/0092-8674(94)90404-9.

Authors

X Jacq¹, C Brou, Y Lutz, I Davidson, P Chambon, L Tora

Affiliation

¹ Laboratoire de Génétique Moléculaire des Eucaryotes, Centre National de la Recherche Scientifique, Faculté de Médecine, Strasbourg, France.

PMID: 7923369
DOI: 10.1016/0092-8674(94)90404-9

Abstract

We showed previously that coactivators mediating stimulation by different activators were associated with the TATA-binding protein (TBP) in distinct TFIID complexes. We have characterized a human TBP-associated factor (TAF), hTAFII30, associated with a subset of TFIID complexes. hTAFII30 interacts with the AF-2-containing region E of the human estrogen receptor (ER), but not with ER AF-1 or VP16. An antibody against hTAFII30 inhibited transcriptional stimulation by the ER AF-2 without affecting basal or VP16-activated transcription and allowed the separation of TFIID complex(es) containing hTAFII30 from complexes mediating the activity of VP16. These results directly demonstrate the existence of functionally distinct TFIID populations that share common TAFIIs but differ in specific TAFIIs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / metabolism
HeLa Cells
Herpes Simplex Virus Protein Vmw65 / metabolism
Histone Acetyltransferases
Humans
Molecular Sequence Data
Nuclear Proteins / metabolism
Peptide Fragments / immunology
Receptors, Estrogen / metabolism*
Recombinant Proteins / biosynthesis
Sequence Analysis
TATA Box
TATA-Binding Protein Associated Factors*
TATA-Box Binding Protein
Transcription Factor TFIID
Transcription Factors / genetics*
Transcription Factors / metabolism*
Transcriptional Activation / physiology*

Substances

DNA-Binding Proteins
Herpes Simplex Virus Protein Vmw65
Nuclear Proteins
Peptide Fragments
Receptors, Estrogen
Recombinant Proteins
TAF10 protein, human
TATA-Binding Protein Associated Factors
TATA-Box Binding Protein
Transcription Factor TFIID
Transcription Factors
Histone Acetyltransferases
TATA-binding protein associated factor 250 kDa

Associated data

GENBANK/U13991