Dissecting the temporal requirements for homeotic gene function

Development. 1994 Jul;120(7):1983-95.

Abstract

Homeotic genes confer identity to the different segments of Drosophila. These genes are expressed in many cell types over long periods of time. To determine when the homeotic genes are required for specific developmental events we have expressed the Ultrabithorax, abdominal-A and Abdominal-Bm proteins at different times during development using the GAL4 targeting technique. We find that early transient homeotic gene expression has no lasting effects on the differentiation of the larval epidermis, but it switches the fate of other cell types irreversibly (e.g. the spiracle primordia). We describe one cell type in the peripheral nervous system that makes sequential, independent responses to homeotic gene expression. We also provide evidence that supports the hypothesis of in vivo competition between the bithorax complex proteins for the regulation of their down-stream targets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / genetics
  • DNA-Binding Proteins / genetics
  • Drosophila / embryology
  • Drosophila / genetics*
  • Drosophila Proteins*
  • Gene Expression / physiology
  • Genes, Homeobox / physiology*
  • Genes, Insect / physiology*
  • Homeodomain Proteins*
  • Insect Hormones / genetics
  • Morphogenesis / genetics
  • Nuclear Proteins*
  • Peripheral Nervous System / embryology*
  • Promoter Regions, Genetic
  • Proteins / genetics
  • Transcription Factors*

Substances

  • Abd-B proteins, Drosophila
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Homeodomain Proteins
  • Insect Hormones
  • Nuclear Proteins
  • Proteins
  • Transcription Factors
  • Ubx protein, Drosophila
  • abd-A protein, Drosophila