Molecular identification of catalases from Nicotiana plumbaginifolia (L.)

FEBS Lett. 1994 Sep 19;352(1):79-83. doi: 10.1016/0014-5793(94)00923-6.

Abstract

We have isolated three different catalase cDNAs from Nicotiana plumbaginifolia (cat1, cat2, and cat3) and a partial sequence of a fourth catalase gene (cat4) that shows no discernible expression based on Northern analysis. The catalase sequences were used to determine the similarity with other plant catalases and to study the transcriptional response to paraquat, 3-aminotriazole, and salicylic acid. 3-Aminotriazole induces mRNA levels of cat1, cat2 and cat3, indicating that a reduction in catalase activity positively affects catalase mRNA abundance. Salicylic acid that binds catalase in vitro, had no effect on catalase transcript levels at physiological concentrations. Paraquat resulted in the induction of cat1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amitrole / pharmacology
  • Base Sequence
  • Catalase / antagonists & inhibitors
  • Catalase / genetics*
  • Catalase / metabolism
  • Chitinases / metabolism
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Free Radical Scavengers
  • Molecular Sequence Data
  • Paraquat / pharmacology
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plants, Toxic*
  • RNA, Messenger / analysis
  • Salicylates / pharmacology
  • Salicylic Acid
  • Sequence Homology, Amino Acid
  • Tobacco / enzymology*
  • Tobacco / genetics
  • Transcription, Genetic

Substances

  • DNA, Complementary
  • Free Radical Scavengers
  • Plant Proteins
  • RNA, Messenger
  • Salicylates
  • Catalase
  • Chitinases
  • Salicylic Acid
  • Paraquat
  • Amitrole

Associated data

  • GENBANK/Z36975
  • GENBANK/Z36976
  • GENBANK/Z36977